Introduction:
Biomanufacturing relies heavily on microbial platforms for efficient production. However, these platforms need to balance maximizing output with minimizing energy inputs in the form of adenosine-5′-triphosphate (ATP). To understand the energy dynamics of microbial metabolism and enhance bioproduction, scientists at Washington University in St. Louis used a genetically encoded ATP biosensor to study ATP levels in different growth phases and carbon sources in various microbial strains.
- The scientists observed transient ATP accumulations during the transition from exponential to stationary growth phases, coinciding with the production of fatty acids and polyhydroxyalkanoates in Escherichia coli and Pseudomonas putida, respectively.
- They identified carbon sources that elevated ATP levels and boosted the production of fatty acids and polyhydroxyalkanoates.
- Supplementing with certain carbon sources, such as acetate for E. coli and oleate for P. putida, resulted in higher ATP levels and enhanced yield of target products.
- The ATP biosensor was also used to assess metabolic burden and identify bottlenecks that limit the production of limonene in E. coli.
- The study provides insights into microbial energy homeostasis, optimization of bioproduction processes, and the impact of ATP levels on microbial product yields.
Conclusion:
The study at Washington University in St. Louis reveals the relationship between ATP dynamics and bioproduction in microbial platforms. By understanding the energy burdens and optimizing carbon sources, scientists can enhance bioproduction processes and identify metabolic bottlenecks. This research has broad implications for various biomanufacturing systems and can contribute to more efficient and sustainable production practices.
