🔍 They tested noninvasive optical and dielectric techniques.
⚙️ These methods matched traditional standards but are automated and less invasive.
📊 PHARMAFLOW™ captures images for analysis, while capacitance probes gauge cell metabolic status.
🚀 Future plans include exploring Raman scattering for further automation in bioprocessing.
Introduction:
The article discusses innovative techniques developed by the National Institute for Bioprocessing Research and Training (NIBRT) in Ireland for noninvasive monitoring of cell viability during bioprocessing. These techniques address the limitations of traditional methods, aiming to improve operational efficiency and data accuracy in the biopharmaceutical industry.
- NIBRT successfully tested two noninvasive techniques for monitoring cell viability: optical imaging and dielectric measurement.
- The new methods were compared to traditional Trypan blue staining, which requires invasive sample extraction and manual handling.
- The PHARMAFLOW system captures digital images of cells, utilizing software to analyze morphological parameters for viability assessment.
- ABER Instruments’ capacitance probe measures changes in cell populations and metabolic status by assessing electrical properties in a sterile environment.
- Future plans include exploring less developed noninvasive techniques like Raman scattering for improved bioprocess monitoring.
Conclusion:
The advancements in cell viability monitoring presented by NIBRT signify a shift towards more efficient and less invasive methodologies in bioprocessing, which could enhance product consistency and reduce operational costs. Ongoing exploration of various technologies may further optimize these processes, presenting new opportunities for automation and real-time monitoring in the biopharmaceutical field.
