🔬 Researchers have developed a method to detect different isoforms of plasmid DNA using microfluidic electrophoresis.
🧬 The method can detect isoforms as small as 0.1 ng/µL for supercoiled and linear isoforms and 0.5 ng/µL for circular isoforms.
💉 This method can help optimize pDNA production processes and ensure the quality of nucleic acid-based vaccines and therapies.
🧪 The scientists developed a microfluidic supercoiled pDNA assay and validated its separation efficacy.
💡 The next step is to quantify the isoforms in terms of size and concentration.
🧬 The method can detect isoforms as small as 0.1 ng/µL for supercoiled and linear isoforms and 0.5 ng/µL for circular isoforms.
💉 This method can help optimize pDNA production processes and ensure the quality of nucleic acid-based vaccines and therapies.
🧪 The scientists developed a microfluidic supercoiled pDNA assay and validated its separation efficacy.
💡 The next step is to quantify the isoforms in terms of size and concentration.
📢 Breakthrough Method Detects Different DNA Forms
Introduction:
This article discusses a new method for assessing different isoforms of plasmid DNA (pDNA) using microfluidic electrophoresis. The researchers developed a high-throughput microfluidic electrophoresis technique to detect supercoiled, linear, and open circular pDNA isoforms. This method can help optimize processes and analyze batches of pDNA.
Main points:
- The demand for pDNA for nucleic acid-based vaccines and therapies is increasing, and it is important to ensure the quality and purity of the pDNA.
- The new method developed by the researchers can detect supercoiled, linear, and open circular pDNA isoforms.
- The method has a high sensitivity and can detect isoforms as small as 0.1 ng/µL for supercoiled and linear isoforms, and 0.5 ng/µL for the circular isoform.
- The researchers optimized the separation of supercoiled and linear isoforms using a 0.10% gel concentration and a 2200 V separation for pDNA samples.
- The method generates consistent results and matches the length of supercoiled or linear plasmids determined by ladders.
Conclusion:
The development of this microfluidic electrophoresis method allows for the accurate assessment of different isoforms of pDNA, which is crucial for ensuring the quality and efficacy of nucleic acid-based vaccines and therapies. Further research is needed to deepen the understanding of these isoforms and enable their quantitation in terms of size and concentration.
