💡 This method simplifies analysis by eliminating the need for lengthy offline testing.
🔬 OTR serves as a real-time proxy for cellular respiration, indicating glucose consumption.
⚙️ The new RAMOS system provides more accurate data, enhancing the production of therapies like monoclonal antibodies.
Introduction:
The study by researchers from RWTH Aachen University presents a novel application of oxygen transfer rate (OTR) measurement in the monitoring of mammalian cell cultures. Traditionally used in microbial fermentations, this method offers a precise approach to assess cell health and activity without the need for lengthy offline analysis processes.
- The research demonstrates a successful application of OTR monitoring for tracking mammalian cell behavior and metabolic activity.
- OTR serves as a proxy for cellular respiration, providing insights into glucose consumption and overall cell health.
- The RAMOS (Respiratory Activity Monitoring System) instrument offers a sensitive and real-time method for measuring OTR in cell cultures.
- Recent publications by the team validate the effectiveness of OTR measurements in improving monitoring precision compared to traditional off-gas analysis methods.
- This innovative technique is expected to enhance product quality assessments in various applications, such as monoclonal antibody production and advanced therapies.
Conclusion:
The findings highlight the potential of OTR monitoring as a transformative tool in the field of cell culture, fostering more accurate and timely assessments of cellular dynamics. This advancement could lead to improved manufacturing processes and product quality for biopharmaceuticals and therapeutic applications in the near future.
