🔍 EBV detection is manageable, but OvHV-2 poses greater challenges due to broader infection potential across species.
🔬 Continuous monitoring during bioprocesses is crucial to mitigate risks, incorporating advanced detection techniques for better outcomes.
Introduction:
The article discusses the critical issue of viral contamination in cell cultures used for bioprocessing, highlighting the threats posed by common viral contaminants, particularly Epstein-Barr virus (EBV) and ovine herpesvirus 2 (OvHV-2). It emphasizes the necessity for effective monitoring strategies to maintain the integrity of cell culture processes in the development of viral treatments.
- Viral contamination in cell cultures can significantly hinder the bioprocessing of viral treatments and requires sophisticated detection methods.
- EBV is a widespread virus that infects around 98% of the human population but is manageable due to established detection techniques like PCR assays.
- OvHV-2 poses a more significant threat as it can infect multiple species and is challenging to monitor, complicating research in biotechnology and pharmaceutical fields.
- Advances in detection, such as high-throughput sequencing and single-cell analysis, may improve the monitoring of EBV and OvHV-2, revealing viral diversity within cultures.
- Continuous vigilance for viral contamination is necessary throughout the entire bioprocess workflow, as infections can arise from contaminated cell lines, materials, or process breakdowns.
Conclusion:
In summary, maintaining viral vigilance is critical in cell culture-based processes, especially regarding the ubiquitous presence of EBV and the challenging nature of OvHV-2. The implementation of advanced monitoring techniques and a comprehensive approach to the entire bioprocess workflow are imperative for ensuring the safety and effectiveness of viral treatment development.
