🌱 Led by Veronique Chotteau, this model simulates metabolic processes in cell culture.
🧬 It aims to improve biomanufacturing through a hybrid approach using computer modeling and biochemistry data.
🔧 Future plans include testing on immune cells and integrating sensors for better process control.
Introduction:
This article discusses a novel metabolism-based model developed by researchers in Sweden for understanding the dynamics of mammalian cell cultures used in antibody production. Led by Professor Veronique Chotteau, the model aims to enhance biomanufacturing processes by simulating metabolic processes and creating a digital twin of cell types typically employed in bioprocessing.
- The model incorporates metabolic reactions, providing a comprehensive view of cell function within bioreactor systems.
- It simulates antibody production processes, detailing the involvement of various amino acids and energy sources in the culture environment.
- Initially developed for perfusion bioreactors, the model is now being adapted for use with fed-batch systems.
- Testing has been conducted with common cell types, such as Chinese Hamster Ovary (CHO) and Human Embryonic Kidney (HEK) cells.
- The future direction includes applying the model to immune cells and enhancing it with sensor integrations for improved process control.
Conclusion:
The metabolism-based model presents significant advancements in the understanding and optimization of mammalian cell culture processes for antibody production. By merging computer modeling with traditional biochemical principles, this approach not only offers insights into cellular kinetics but also sets the groundwork for integrating real-time process feedback through sensor technology. Future developments could further revolutionize biomanufacturing practices, ultimately improving efficiency and accuracy in the field.
