Introduction:
Protein-protein interactions play a crucial role in many biological functions and understanding these interactions is key to understanding physiological processes in health and disease. Protein science and interaction methods are widely used in biologic and medicinal research, forming the foundation of pharmacological theory. Modern microplate readers have revolutionized protein-protein interaction studies by providing various measurement methods to study these interactions.
- Microplate readers have enabled the study of protein-protein interactions by providing various measurement methods.
- Fluorescence- or luminescence-based measurements are commonly used due to their higher sensitivity and high-throughput compatibility.
- Methods based on Förster’s Resonance Energy Transfer (FRET) and Bioluminescence Resonance Energy Transfer (BRET) are commonly employed to detect protein-protein interactions.
- Advanced applications like NanoBRET and TR-FRET have been developed to improve sensitivity and readout quality.
- Microplate readers, like BMG LABTECH’s PHERAstar® FSX, provide the sensitivity and speed required for high-throughput protein-protein interaction studies.
Conclusion:
Microplate readers have revolutionized the study of protein-protein interactions and have enabled researchers to obtain valuable insights into the binding kinetics and stoichiometry of these interactions. Advanced methods like NanoBRET and TR-FRET have improved sensitivity and readout quality. Microplate readers, like BMG LABTECH’s PHERAstar® FSX, provide the necessary performance and features for high-throughput protein-protein interaction studies.
