🚨 These biomarkers can be detected within 48 hours, providing an early warning system.
❗️ Other methods, like immunofluorescence and RNA-based assays, have limitations and are time-consuming.
✅ The relative abundance ratios of specific proteins were found to be a reliable indicator of pluripotency loss.
🔬 Monitoring protein biomarkers can be used in commercial applications and research labs.
Introduction:
Protein biomarkers have been identified that can indicate the imminent loss of pluripotency in human pluripotent stem cell (hPSC) cultures. These biomarkers can be detected within the first 48 hours after pluripotency dissolution begins, providing an early warning system for changes in hPSCs’ pluripotency. This discovery overcomes current limitations in monitoring pluripotency, such as subjective cell morphology assessments and time-consuming RNA-based assays.
- Monitoring protein biomarkers secreted in hPSC cultures can detect changes in pluripotency.
- Immunofluorescence, flow cytometry, RNA-based assays, and cell morphology assessments are currently used to monitor pluripotency but have limitations.
- An analysis of the proteomic and transcriptomic changes of hPSC cultures identified protein biomarkers indicating pluripotency loss.
- The relative abundance ratios of specific proteins were found to be more reliable indicators of pluripotency loss than changes in individual biomarkers.
- The identified biomarkers have potential applications in commercial scale-up settings and research labs for monitoring pluripotency.
Conclusion:
The identification of protein biomarkers that can indicate the imminent loss of pluripotency in hPSC cultures is a significant advancement in stem cell research and development. These biomarkers provide a fast and non-invasive method for monitoring pluripotency and overcoming current limitations in assessing pluripotent cell cultures. The use of protein biomarkers has potential implications in various applications, including drug discovery and stem cell-based therapies.
