🔬 ddPCR is a targeted, high-throughput, and cost-effective alternative to RNA sequencing applications.
💯 The absolute copy numbers quantified by ddPCR were comparable to other methods, with a coefficient of variation of only five percent.
🧪 ddPCR can be used for targeted transcript quantification, screening for host cell proteins, and predicting screening clones.
💡 Combining ddPCR with amplitude multiplexing may improve scalability, efficiency, and throughput.
Introduction:
Droplet digital polymerase chain reaction (ddPCR) technology is being used as a method to measure transgene copy numbers and assess copy number stability in clonal cell lines. This method is accurate, efficient, and cost-effective, making it a valuable tool in cell line development and monitoring.
- ddPCR is emerging as a preferred method for assessing copy number stability in clonal cell lines.
- The absolute copy numbers quantified by ddPCR are comparable to those identified by other methods.
- ddPCR can be used for targeted transcript quantification and screening for host cell proteins or genes.
- Combining ddPCR with amplitude multiplexing can improve scalability, efficiency, and throughput.
- A new one-step ddPCR device is being developed to overcome current instrument limitations.
Conclusion:
ddPCR is a valuable tool for monitoring cell line stability and quantifying transgene copy numbers. Its accuracy and efficiency make it a preferred method for clonal cell line assessment. Further advancements in ddPCR technology, such as the development of one-step devices, have the potential to improve scalability and reduce workflow and costs.
