🔍 A recent study compared filters from three manufacturers and found that larger internal diameters and shorter filter lengths reduce fouling.
💡 Filters with 0.2 μm pore size and 2.6 mm internal diameter maintained high product sieving and low hydraulic resistance.
⚙️ The study suggests that optimizing the design of hollow fiber filters can help maximize membrane area and reduce fouling. 🧪
Introduction:
This article discusses the optimization of hollow fiber filters for maximum membrane area in order to overcome fouling in perfusion cell cultures. The fouling of hollow fiber filter membranes can lead to unstable processes and process failures in continuous manufacturing. The article highlights a recent study that compares three manufacturers’ filters in terms of fouling and evaluates the impact of pore size, inner diameter, and filter length on product retention and transmembrane pressure in tangential flow filtration (TFF) perfusion cell culture.
- The study compared hollow fiber filters from three manufacturers in terms of fouling and assessed the effects of pore size, inner diameter, and filter length on product retention and transmembrane pressure in TFF perfusion cell culture.
- Filters with larger internal diameters were found to maintain higher product sieving and lower hydraulic membrane resistance.
- Shorter length hollow fibers and larger lumen ID, as well as lower shear rate, were found to result in a significant reduction in fouling.
- Pore size was also found to affect both sieving and hydraulic membrane resistance, with larger pore sizes reducing both parameters.
- The study indicates that optimizing hollow fiber filters for maximum membrane area can help overcome fouling and improve the stability and efficiency of perfusion cell cultures in continuous manufacturing processes.
Conclusion:
This study highlights the importance of optimizing hollow fiber filters for maximum membrane area to minimize fouling in perfusion cell cultures. By considering factors such as pore size, inner diameter, and filter length, researchers can improve product retention, reduce hydraulic membrane resistance, and achieve more stable and efficient processes in continuous manufacturing. Further research in this area can help advance the development of continuous manufacturing technologies for biopharmaceutical production.
