🌍 They used single compounds like glucose, achieving accurate results comparable to real processes.
💡 This method could expedite analytics in process development, especially for small companies.
📈 Upcoming research will explore synthetic data and different cell lines.
🚀 The goal is to enhance analytical techniques for early-stage development!
Introduction:
This article discusses recent advancements in calibrating Raman spectroscopy, a widely used analytical technique, without the necessity of operating a bioreactor. Conducted by researchers at Delft University of Technology in the Netherlands, the study highlights an innovative approach that utilizes single compounds to calibrate Raman models more efficiently and cost-effectively than traditional methods.
- The research team established that Raman spectroscopy could be calibrated using single compounds, bypassing the costly and time-consuming bioreactor processes.
- Dr. Marieke Klijn noted that this breakthrough could facilitate earlier use of analytical technologies in the process development phases for researchers who may lack resources.
- The study involved running batch and fed-batch processes in a bioreactor and analyzing sixteen single compound measurements to mimic yeast fermentation.
- Despite increased computational demands, the new calibration technique significantly reduced the number of experiments required for accurate modeling.
- Future work aims to explore synthetic data generation and assess the method’s applicability across different media and cell lines, including animal cell culture analysis.
Conclusion:
The findings of this study present a substantial advancement in the practical application of Raman spectroscopy for biochemical analysis. The ability to calibrate this technology without operating a bioreactor not only reduces costs and resources but also provides broader access to analytical tools, thereby enhancing research capabilities, particularly for smaller firms and early-stage projects. Continued exploration in this area will likely lead to further innovations in analytical chemistry and process development.
